Partial purification and characterization of a trypsin inhibitor isolated from Adenanthera pavonina L. seeds
نویسندگان
چکیده
منابع مشابه
The oil of Adenanthera pavonina L. seeds and its emulsions.
The oil of Adenanthera pavonina L. seeds was analysed by chromatographic and instrumental means. The oil was found to be rich in neutral lipids (86.2%), and low in polar lipids (13.8%). The neutral lipids consisted mainly of triacylglycerols (64.2%). Unsaturated fatty acids were found as high as 71%, while the percentage of saturated fatty acids was only 29%. GC and GC/MS analyses revealed lino...
متن کاملPhysiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds.
We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds...
متن کاملPurification, characterization, and complete amino acid sequence of a trypsin inhibitor from amaranth (Amaranthus hypochondriacus) seeds.
A protein proteinase inhibitor was purified from a seed extract of amaranth (Amaranthus hypochondriacus) by precipitation with (NH4)2SO4, gel-filtration chromatography, ion-exchange chromatography, and reverse-phase high-performance liquid chromatography. It is a 69-amino acid protein with a high content of valine, arginine, and glutamic acid, but lacking in methionine. The inhibitor has a rela...
متن کاملGenomic and cDNA cloning, expression, purification, and characterization of chymotrypsin-trypsin inhibitor from winged bean seeds.
A 516-bp winged bean chymotrypsin-trypsin inhibitor (WbCTI) gene was amplified from genomic DNA and cDNA isolated from winged bean using a pair of degenerate primers designed on the basis of the amino acid sequences of WbCTI. The amplified PCR products were cloned and sequenced to confirm their authenticity. DNA sequence analysis of the genomic and cDNA clones of WbCTI revealed the same nucleot...
متن کاملPartial purification of the serum trypsin inhibitor.
The serum trypsin inhibitor has been studied by many investigators since Lansteiner (1) associated it with the albumin fraction by salt fractionation as did Smith and Lindsley (2) by the electrophoretic separation of serum. Both Christensen and MacLeod (3), and MacFarlane and Pilling (4), also associated the trypsin inhibitor with the albumin fraction but differentiated it from the plasmin inhi...
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ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2016
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2015.11.008